Determination of 3-hydroxyanthranilic acid in the sweat of healthy older adults.

     3-Hydroxyanthranillic acid (3HAA) is a substance made during tryptophan metabolism and is linked to aging and age-related diseases.
This study developed a simple and fast method to detect 3HAA in sweat using HPLC. The method can detect 3HAA, along with other related compounds (KYN and TRP), in under 6 minutes and showed high accuracy and precision. In a study with 81 healthy adults aged 50–79, 3HAA was found in all sweat samples — the first time it’s been reported in human sweat. Levels of 3HAA increased with age. These results help us better understand how sweat can reflect aging and could support early diagnosis and personalized care for older adults.