Pyruvate carboxylase (PC) (EC 6.4.1.1) is a biotin-dependent carboxylase which catalyzes the ATP-dependent carboxylation of pyruvate to form oxaloacetate which is an important precursor for several metabolic pathways including gluconeogenesis, lipogenesis, neurotransmitter synthesis and glucose-induced insulin secretion from pancreatic Read More …
The tricarboxylic acid (TCA) cycle functions as both an oxidative hub to oxidize acetyl-CoA and a biosynthetic hub for the synthesis of nucleotides, amino acids and fatty acids. Glutaminloysis and pyruvate carboxylation are important anaplerotic reactions which maintain Read More …
Metabolic reprograming in cancers is one of cancer hallmarks that allow cancer cell to expand and metastasize to other organs. In collaboration with Assoc Prof Kulthida Vaeteewoottacharn’s research team at Department of Medicine, Khon-Kaen University, they found that the acetyl-CoA Read More …
Brown adipose tissue or BAT is the adipocytes that have a specific physiology in maintaining body temperature via thermogenesis. BAT activity is mainly regulated by the sympathetic nervous system, which stimulates the thermogenic response while simultaneously increasing the Read More …
Metabolic reprograming in cancers is one of cancer hallmarks that allow cancer cell to expand and metastasize to other organs. Professor Sarawut Jitrapakdee and his research team have recently shown that the holocarboxylase synthetase (HLCS) enzyme, a Read More …
BC Faculty Seminar “Holocarboxylase synthetase as a novel anti-cancer target” by Prof. Sarawut Jitrapakdee Department of Biochemistry, Faculty of Science Mahidol University
Overexpression of Holocarboxylase Synthetase Predicts Lymph Node Metastasis and Unfavorable Prognosis in Breast Cancer Witchuda Sukjoi1, Siraprapa Siritutsoontorn1, Pakkanan Chansongkrow1, Suppakit Waiwitlikhit1,2, Steven W. Polyak3, Malee Warnnissorn4, Varodom Charoensawan1,5,6,Chanitra Thuwajit7, Sarawut Jitrapakdee1* 1Department of Biochemistry, Faculty of Science, Mahidol University, Read More …
Overexpression of Pyruvate Carboxylase Is Correlated With Colorectal Cancer Progression and Supports Growth of Invasive Colon Cancer HT-29 Cell Line Jarunya Ngamkham1, Chanitra Thuwajit2, Peti Thuwajit2, Peerapat Khamwachirapitha3, Kornkamon Lertsuwan3, Varodom Charoensawan3,4,5 and Sarawut Jitrapakdee3 1Graduate Program in Read More …
Characterization of intra- and inter-species hybrid tetramers of pyruvate carboxylase: Biotin and the BCCP domain play a crucial role in determination of the kinetics and thermodynamics of catalysis Khanti Rattanapornsompong, Sarawut Jitrapakdee1 and Paul V. Attwood2 1Department of Biochemistry, Faculty Read More …