The role of the phosphate groups of trinitrophenyl adenosine 5′-triphosphate (TNP-ATP) in allosteric activation of pyruvate carboxylase and the inhibition of acetyl CoA-dependent activation

Sarawut Jitrapakdee

          Pyruvate carboxylase (PC) (EC 6.4.1.1) is a biotin-dependent carboxylase which catalyzes the ATP-dependent carboxylation of pyruvate to form oxaloacetate which is an important precursor for several metabolic pathways including gluconeogenesis, lipogenesis, neurotransmitter synthesis and glucose-induced insulin secretion from pancreatic Read More …

Overexpression of Holocarboxylase Synthetase Predicts Lymph Node Metastasis and Unfavorable Prognosis in Breast Cancer

Sarawut Jitrapakdee

Overexpression of Holocarboxylase Synthetase Predicts Lymph Node Metastasis and Unfavorable Prognosis in Breast Cancer Witchuda Sukjoi1, Siraprapa Siritutsoontorn1, Pakkanan Chansongkrow1, Suppakit Waiwitlikhit1,2, Steven W. Polyak3, Malee Warnnissorn4, Varodom Charoensawan1,5,6,Chanitra Thuwajit7, Sarawut Jitrapakdee1* 1Department of Biochemistry, Faculty of Science, Mahidol University, Read More …

Overexpression of Pyruvate Carboxylase Is Correlated With Colorectal Cancer Progression and Supports Growth of Invasive Colon Cancer HT-29 Cell Line

Sarawut Jitrapakdee

Overexpression of Pyruvate Carboxylase Is Correlated With Colorectal Cancer Progression and Supports Growth of Invasive Colon Cancer HT-29 Cell Line   Jarunya Ngamkham1, Chanitra Thuwajit2, Peti Thuwajit2, Peerapat Khamwachirapitha3, Kornkamon Lertsuwan3, Varodom Charoensawan3,4,5 and Sarawut Jitrapakdee3   1Graduate Program in Read More …

Characterization of intra- and inter-species hybrid tetramers of pyruvate carboxylase: Biotin and the BCCP domain play a crucial role in determination of the kinetics and thermodynamics of catalysis

Sarawut Jitrapakdee

Characterization of intra- and inter-species hybrid tetramers of pyruvate carboxylase: Biotin and the BCCP domain play a crucial role in determination of the kinetics and thermodynamics of catalysis Khanti Rattanapornsompong, Sarawut Jitrapakdee1 and Paul V. Attwood2 1Department of Biochemistry, Faculty Read More …