CRISPR/Cas9-mediated double knockout of SRPK1 and SRPK2 in a nasopharyngeal carcinoma cell line

        Nasopharyngeal carcinoma (NPC) originates from the epithelial cells of the nasopharynx. A high incidence is found in China and the Southeast Asia including Thailand. Serine-arginine protein kinase (SRPK) is a regulator of alternative splicing events via phosphorylation of splicing factor proteins. Oncogenic roles of SRPK1 and SRPK2 have been reported in various types of cancer. Here we aimed to establish a nasopharyngeal carcinoma (NPC) cell line with stable SRPK1 or SRPK2 knockout and SRPK1/2 double knockout as a model to investigate their potential roles in NPC. These NPC cell lines pose as a model for investigation of their splicing mechanism in NPC.

Reference:

Prattapong P, Ngernsombat C, Aimjongjun S, Janvilisri T. CRISPR/Cas9-mediated double knockout of SRPK1 and SRPK2 in a nasopharyngeal carcinoma cell line. Cancer Rep (Hoboken). 2020 Apr;3(2):e1224. doi: 10.1002/cnr2.1224. Epub 2019 Nov 27. PMID: 32671994; PMCID: PMC7941449.

DOI: 10.1002/cnr2.1224