Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase

Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase

 

Research_RN

 

Bacterial luciferase catalyzes bioluminescent reaction that has long been used for sensitive detection tools in biomedical, food, and environmental applications. In this research, we elucidated crucial factor to control the protonation of intermediate that determines the light output of bacterial luciferase reaction. Our finding showed that the bioluminescent reaction proceeds by generating a reactive intermediate of flavin C4a-oxygen adduct and the intermediate is first generated in a protonated form of flavin C4a-hydroperoxide, which is unable to react with an aldehyde. The active site His44 functions as an essential proton abstractor to convert the flavin C4a-hydroperoxide to flavin C4a-peroxide that is capable to proceed the bioluminescent reaction. This is the first identification of mechanistic control of the protonation state of flavin intermediates in a nucleophilic flavin‐dependent monooxygenase. Understanding how the protonation of flavin C4a‐OOH can be controlled is important for fine‐tuning the activity of nucleophilic or electrophilic monooxygenases.

 

Reference:

Tinikul R, Lawan N,  Akeratchatapan N, Pimviriyakul P,  Chinantuya W, Suadee C, Sucharitakul J,  Chenprakhon P,  Ballou DP, Entsch B, Chaiyen P. Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase. FEBS J. https://doi.org/10.1111/febs.15653

 

Relevant SDGs
SDGs_Goal-3
BC investigator
Ruchanok Tinikul Asst. Prof. Ruchanok Tinikul