Cholangiocarcinoma, a bile duct cancer, is highly prevalent in Northeast, Thailand and is associated with liver fluke infection. It is an aggressive cancer with high mortality rate, partly due to late detection. Therefore, it is a very important public health problem in this country. p53, a tumor suppressor, is the most mutated gene in human cancers including liver fluke-associated cholangiocarcinoma (CCA). Hence, expression patterns of p53 and its target genes may be useful for diagnosis and prognosis of CCA patients. Here the differential mRNA expression of p53 and p53 target genes in CCA and adjacent non-cancerous tissues were investigated. mRNA expression of p53 target genes were downregulated, while p53 protein was elevated in most of CCA tissues indicating that some p53 should be non-functional mutation p53. Down-regulation of WIP1 statistically significant associated with non-papillary type. In addition, PAI-1 mRNA was down-regulated in almost all CCA tissue samples, highlighting it as potential diagnostic marker of CCA.
Puetkasichonpasutha J, Namwat N, Sa-Ngiamwibool P, Titapun A, Suthiphongchai T. Evaluation of p53 and Its Target Gene Expression as Potential Biomarkers of Cholangiocarcinoma in Thai Patients. Asian Pac J Cancer Prev. 2020 Mar 1; 21(3):791-798. doi: 10.31557/APJCP.2020.21.3.791
|Assoc. Prof. Tuangporn Suthiphongchai
|Ms. Janpen Puetkasichonpasutha