The measurement of cellular metabolites is crucial for medical applications as specific biomarkers can serve as disease indicators, while the detection of microbial metabolites in metabolic engineered cells applied in advanced biotechnology is useful for optimizing bioconversion efficiency. Enzyme-based detection has long been applied as a sensitive and specific methodology for measuring target compounds because it offers unique advantages in terms of high molecular recognition, sensitive due to carrying multiple turnover ability to amplify signals, and enzymatic properties can be improved by protein engineering to obtain better variants. In addition, enzymes can be utilized in the form of single or multistep enzyme cascades to give a variety of reading signals. This review provides a critical discussion of redox enzymatic cascades currently employed for detection of human- and microbe-related cellular metabolites. The reaction prototypes mentioned in this review can further be developed to be cost-effective, portable, and user-friendly devices for human health and bioprocess assessments.
Tinikul R, Trisrivirat D, Chinantuya W, Wongnate T, Watthaisong P, Phonbuppha J, Chaiyen P. Detection of cellularmetabolites by redox enzymatic cascades. Biotechnol. J. 2022, 17, 2100466.
|Asst. Prof. Ruchanok Tinikul